AKTA Protein Purification
System

General Requirements: Protein Purification Act: AKTA PRIME PLUS AKTA Prime Plus is a chromatography instrument for routine protein purification in research laboratories. The flexible and intuitive chromatography system accelerates the protein purification process from microgram to gram target product concentrations. 

Sample Requirements for Genomics Laboratories:
1. Enzyme/protein samples have undergone a specific size selection process using dialysis membranes.
2. Enzyme/protein samples and buffers have been pre-filtered using 0.22 μL membrane filters.
3. Columns, buffers, and sample tubing are provided by the user operating the AKTA system.
4. Contact the AKTA equipment coordinator before submitting a request via ELSA.
5. Users operating the AKTA Prime Plus must have experience in protein purification. If inexperienced, they must be accompanied by a colleague familiar with enzyme purification equipment.
6. The same type of enzyme can be run in one day with the same ELSA ID (the price will be adjusted according to the number of samples). The service price is IDR 350,000/sample (maximum 6 hours); if the samples are different, a new ELSA ID must be submitted.

Notes:

1. The new service is open to BRIN internal staff who are qualified to operate the equipment.

2. The equipment may only be operated by civil servants, and students must be accompanied by a civil servant during use.

3. Please provide sample information in the sample description section. 4. For sample analyses exceeding 5 mL, confirmation must be obtained from the administrator and noted in the service description section during submission.

AKTA Go is a chromatography instrument for routine protein purification in research laboratories. (Source: https://www.cytivalifesciences.com/en/us/shop/chromatography/chromatography-systems/akta-go-protein-purification-system-p-11219).

A flexible and intuitive chromatography system to accelerate the protein purification process from target product concentrations at the microgram level up to grams.

Buffers and columns are provided by the service user.

SPECIAL NON-MEDICAL/NON-INFECTIOUS SAMPLES

• Enzyme/protein samples have undergone a specific size selection process using a dialysis membrane.

• Enzyme/protein samples and buffers have been pre-filtered using a 0.22 μL membrane filter

• Columns, buffers, and sample tubing are provided by the user operating the AKTA system

• Contact the Genomic Lab Service Number before submitting a request via ELSA, mobile number (+62 811-9811-575)

• Users who will use AKTA Pure must have experience in protein purification. If they do not have experience, they must be accompanied by a colleague who is familiar with using enzyme purification equipment

 The same type of enzyme can be processed in one day using the same ELSA ID (pricing will be adjusted based on the number of samples). Service fee: IDR 350,000 per sample (maximum 6 hours). If the samples are different, a new ELSA ID must be submitted.

Notes:

1. The service is currently available only for internal BRIN staff who can operate the equipment.

2. Users who submit requests will be designated as service providers and must submit a report, which will be reviewed by the equipment supervisor, within a maximum of 3 days after testing, using the report format available at the following link: https://docs.google.com/document/d/1Ibs7Hwo22K78vH3of7kNDxDNxeMx4Ra9/edit? usp=drive_link&ouid=103985831449687786721&rtpof=true&sd=true.

3. The equipment may only be operated by civil servants, and students must be accompanied by a civil servant during use.

4. Please provide sample information in the sample description section.

5. For sample analysis exceeding 5 mL, confirmation must be obtained from the administrator (Phone: 085717711891) and noted in the service description section during submission.

Compact liquid chromatography system to support routine protein purification in research laboratories. Quickly getting enough protein of desired purity and yield is essential to advance research tasks and get to reliable results. The compact system, with a footprint of only 335 × 464 mm, lets you more efficiently use precious bench and cold cabinet space in your routine column chromatography purifications. (Source: https://www.cytivalifesciences.com/en/us/shop/chromatography/chromatography-systems/akta-go-protein-purification-system-p-11219).

Application Requirements:

• Photo
• Other Supporting File

File Name	File Size
Service SOP/Form file	0.15 MB
Template Photo	0 MB
Template Other Supporting File	0 MB