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SERVICE DESCRIPTION | Antioxidant DPPH method Ø Method: modification of the method of Yen, G.C., and Chen, H.Y., "Antioxidant activity of various tea extracts in relation to heir antimutagenicity," J Agric Food Chem 43: 27-32,1995. Ø Prinsif test: DPPH (2,2-diphenyl-1-picril-hydrazil-hydrate)…
- Laboratorium Cara Pembuatan Obat Tradisional yang Baik (CPOTB)
- Puspiptek - Serpong
Laboratorium Cara Pembuatan Obat Tradisional yang Baik (CPOTB) Gedung 450 Kawasan Sains dan Teknologi BJ. Habibie (PUSPIPTEK) Serpong, 15314, Tangerang Selatan, Banten – Indonesia - 08119811565
- labcpotb@brin.go.id
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Antioxidant DPPH method
Ø Method: modification of the method of Yen, G.C., and Chen, H.Y., "Antioxidant activity of various tea extracts in relation to heir antimutagenicity," J Agric Food Chem 43: 27-32,1995.
Ø Prinsif test: DPPH (2,2-diphenyl-1-picril-hydrazil-hydrate) is a radical which when dissolved in a methanol solvent will be colored violet with absorption at a wavelength (λ) 515 nm. Antioxidant testing is based on electron transfer from analytes (samples containing antioxidants) to DPPH which causes a decrease in the intensity of the DPPH violet color. The more active the sample, the violet color will fade. The antioxidant activity of the sample is calculated based on the difference in absorbance of the blank solution (only DPPH) and the sample solution + DPPH.
Ø Using the DPPH test provides an easy and fast way to evaluate antioxidants with spectrophotometry so that it can be useful for assessing various products at a time.
Application Requirements:
Ø Method: modification of the method of Yen, G.C., and Chen, H.Y., "Antioxidant activity of various tea extracts in relation to heir antimutagenicity," J Agric Food Chem 43: 27-32,1995.
Ø Prinsif test: DPPH (2,2-diphenyl-1-picril-hydrazil-hydrate) is a radical which when dissolved in a methanol solvent will be colored violet with absorption at a wavelength (λ) 515 nm. Antioxidant testing is based on electron transfer from analytes (samples containing antioxidants) to DPPH which causes a decrease in the intensity of the DPPH violet color. The more active the sample, the violet color will fade. The antioxidant activity of the sample is calculated based on the difference in absorbance of the blank solution (only DPPH) and the sample solution + DPPH.
Ø Using the DPPH test provides an easy and fast way to evaluate antioxidants with spectrophotometry so that it can be useful for assessing various products at a time.
Application Requirements:
- Photo
- Other Supporting File
File Name | File Size | |
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Service SOP file | 0.44 MB | |
Template Photo | 0 MB | |
Template Other Supporting File | 0 MB |