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SERVICE DESCRIPTION | Determination of total phenolic compound content in extracts (Total Phenolic Content-TPC). Method: Folin-Ciocalteau (FC) colorimetry is based on the chemical reduction of reagents, a mixture of tungsten and molybdenum oxide based on the method of Singleton,…
- Laboratorium Cara Pembuatan Obat Tradisional yang Baik (CPOTB)
- KST Serpong (Bacharuddin Jusuf Habibie)
Laboratorium Cara Pembuatan Obat Tradisional yang Baik (CPOTB) Gedung 450 Kawasan Sains dan Teknologi BJ. Habibie (PUSPIPTEK) Serpong, 15314, Tangerang Selatan, Banten – Indonesia - 08119811565
- labcpotb@brin.go.id
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Determination of total phenolic compound content in extracts (Total Phenolic Content-TPC). Method: Folin-Ciocalteau (FC) colorimetry is based on the chemical reduction of reagents, a mixture of tungsten and molybdenum oxide based on the method of Singleton, V.L., Orthofer, R., and Lamuela- Raventos, R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent.
Working principle: The total phenolic compound content in the extract is expressed as gallic acid equivalent (mg/g extract) based on the regression equation of the gallic acid calibration curve. The sample solution (250 μl), standard gallic acid solution (0, 10, 50, 100, and 200 μL) were pipetted into a test tube, then added to 4 mL by adding distilled water and 250 μl Folin-Ciocalteau, the mixture was then shaken until homogeneous. After remaining for 8 minutes, 750 μl of 20% Na2CO3 was added and shaken homogeneously. The mixture was then left for 2 hours at room temperature. Absorption was measured at a wavelength of 765 nm. The measurements were carried out 3 times in repetition so that the phenol content obtained was obtained as equivalent gallic acid (μg/mL sample). The intensity of the blue color formed is proportional to the total phenol content in the sample.
Application Requirements:
Working principle: The total phenolic compound content in the extract is expressed as gallic acid equivalent (mg/g extract) based on the regression equation of the gallic acid calibration curve. The sample solution (250 μl), standard gallic acid solution (0, 10, 50, 100, and 200 μL) were pipetted into a test tube, then added to 4 mL by adding distilled water and 250 μl Folin-Ciocalteau, the mixture was then shaken until homogeneous. After remaining for 8 minutes, 750 μl of 20% Na2CO3 was added and shaken homogeneously. The mixture was then left for 2 hours at room temperature. Absorption was measured at a wavelength of 765 nm. The measurements were carried out 3 times in repetition so that the phenol content obtained was obtained as equivalent gallic acid (μg/mL sample). The intensity of the blue color formed is proportional to the total phenol content in the sample.
Application Requirements:
- Photo
- Other Supporting File
| File Name | File Size | |
|---|---|---|
| Service SOP file | 1.12 MB | |
| Template Photo | 0 MB | |
| Template Other Supporting File | 0 MB |
