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SERVICE DESCRIPTION | • Determination of total phenolic compounds in the extract (Total Phenolic Content-TPC) Ø Method: Folin-Ciocalteau (FC) colorimetry is based on chemical reduction of reagents, mixture of tungsten and molybdenum oxide based on the method of Singleton,…
- Laboratorium Cara Pembuatan Obat Tradisional yang Baik (CPOTB)
- Puspiptek - Serpong
Laboratorium Cara Pembuatan Obat Tradisional yang Baik (CPOTB) Gedung 450 Kawasan Sains dan Teknologi BJ. Habibie (PUSPIPTEK) Serpong, 15314, Tangerang Selatan, Banten – Indonesia - 08119811565
- labcpotb@brin.go.id
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• Determination of total phenolic compounds in the extract (Total Phenolic Content-TPC)
Ø Method: Folin-Ciocalteau (FC) colorimetry is based on chemical reduction of reagents,
mixture of tungsten and molybdenum oxide based on the method of Singleton, V.L., Orthofer, R., and Lamuela-Raventos, R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Enzymol Methods. 299: 152-178.
Ø Prinsif action: The total phenolic compound content in the extract is expressed as equivalent gallic acid (mg / g extract) based on the regression equation of the gallic acid calibration curve. Sample solution (250 μl), gallic acid standard solution (0, 10, 50, 100, and 200 μL) pipetted into a test tube, then stirred to 4 mL by adding distilled water and 250 μl Folin-Ciocalteau, the mixture then shaken homogeneously. After settling for 8 minutes, 750 μl 20% Na2CO3 is added and shaken homogeneously. The mixture is then allowed to stand for 2 hours at room temperature. Uptake is measured at a wavelength of 765 nm. Measurements were made 3 times so that the phenol content obtained was obtained as equivalent gallic acid (μg / mL sample). The intensity of the blue color formed is proportional to the total phenol content in the sample.
Application Requirements:
Ø Method: Folin-Ciocalteau (FC) colorimetry is based on chemical reduction of reagents,
mixture of tungsten and molybdenum oxide based on the method of Singleton, V.L., Orthofer, R., and Lamuela-Raventos, R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent. Enzymol Methods. 299: 152-178.
Ø Prinsif action: The total phenolic compound content in the extract is expressed as equivalent gallic acid (mg / g extract) based on the regression equation of the gallic acid calibration curve. Sample solution (250 μl), gallic acid standard solution (0, 10, 50, 100, and 200 μL) pipetted into a test tube, then stirred to 4 mL by adding distilled water and 250 μl Folin-Ciocalteau, the mixture then shaken homogeneously. After settling for 8 minutes, 750 μl 20% Na2CO3 is added and shaken homogeneously. The mixture is then allowed to stand for 2 hours at room temperature. Uptake is measured at a wavelength of 765 nm. Measurements were made 3 times so that the phenol content obtained was obtained as equivalent gallic acid (μg / mL sample). The intensity of the blue color formed is proportional to the total phenol content in the sample.
Application Requirements:
- Photo
- Other Supporting File
| File Name | File Size | |
|---|---|---|
| Service SOP file | 0.44 MB | |
| Template Photo | 0 MB | |
| Template Other Supporting File | 0 MB |
