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SERVICE DESCRIPTION | POLYMERASE CHAIN REACTION (PCR) – SensQuest Service Description The SensQuest Polymerase Chain Reaction (PCR) system is used for the in vitro amplification of specific DNA sequences through controlled temperature cycling. The instrument can be…

  • iLaB (Integrated Laboratory of Bioproducts) - ORNM
  • KST Cibinong (Soekarno)
    KST Ir Soekarno
  • 087884931709
  • maya007@brin.go.id
  • Service Unit: Contractual (per hour service)
  • Service Duration: 1 Hours
  • Service Quota Per Day: 4
  • Service Capacity: 1 Contractual (per hour service)
  • Minimum Amount: 1
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POLYMERASE CHAIN REACTION (PCR) – SensQuest
Service Description

The SensQuest Polymerase Chain Reaction (PCR) system is used for the in vitro amplification of specific DNA sequences through controlled temperature cycling. The instrument can be used to amplify target DNA for various molecular biology and genetic analysis applications.

Service

Use of the SensQuest PCR instrument for DNA amplification according to the PCR program and reaction conditions specified by the user.

Service Specifications
  • Equipment: Polymerase Chain Reaction (PCR) Thermal Cycler
  • Brand: SensQuest
  • Function: Amplification of specific DNA sequences using PCR
  • Sample: Purified DNA template ready for PCR
  • Sample Preparation: Prepared by the user; DNA extraction and purification are not included
  • PCR Reagents: Provided by the user, including DNA template, primers, PCR master mix, and other required reaction components
  • PCR Parameters: Denaturation, annealing, extension, number of cycles, final extension, and other conditions are determined by the user
  • Service Output: Amplified PCR product
  • Operation: Performed by the user according to the applicable SOP and under laboratory staff supervision
Conditions of Use
  1. The user must provide information regarding the sample type, target DNA, and PCR program.
  2. The DNA template must be previously extracted and purified and have suitable quality and concentration for PCR.
  3. All required PCR reagents and consumables must be provided by the user unless otherwise specified by the laboratory.
  4. PCR tubes or plates must be compatible with the SensQuest PCR instrument.
  5. The user must provide the required PCR program, including denaturation, annealing, extension, number of cycles, and final extension conditions.
  6. Appropriate procedures must be followed to prevent cross-contamination between samples and PCR reagents.
  7. The PCR workspace and equipment must be kept clean before and after use.
  8. PCR products must be handled and disposed of according to applicable laboratory procedures.
  9. Users must follow all applicable SOPs, laboratory safety procedures, and equipment operating guidelines.
  10. The user is responsible for the quality, suitability, and safety of the samples and reagents used.


File Name File Size
Service SOP/Form file 0.52 MB