PCR EPPENDORF ASTERCYCLER X50s Access Service
Paid


SERVICE DESCRIPTION | POLYMERASE CHAIN REACTION (PCR) – Eppendorf Mastercycler X50s Service Description The Eppendorf Mastercycler X50s is a PCR thermal cycler used for the in vitro amplification of DNA through controlled temperature cycles. The instrument is suitable…

  • iLaB (Integrated Laboratory of Bioproducts) - ORNM
  • KST Cibinong (Soekarno)
    KST Ir Soekarno
  • 087884931709
  • maya007@brin.go.id
  • Service Unit: Contractual (per hour service)
  • Service Duration: 1 Hours
  • Service Quota Per Day: 5
  • Service Capacity: 1 Contractual (per hour service)
  • Minimum Amount: 1
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POLYMERASE CHAIN REACTION (PCR) – Eppendorf Mastercycler X50s
Service Description

The Eppendorf Mastercycler X50s is a PCR thermal cycler used for the in vitro amplification of DNA through controlled temperature cycles. The instrument is suitable for amplifying specific DNA target sequences for subsequent molecular analysis.

Service

Use of the Eppendorf Mastercycler X50s for DNA amplification using the Polymerase Chain Reaction (PCR) technique according to the PCR program and reaction conditions specified by the user.

Service Specifications
  • Equipment: PCR Thermal Cycler
  • Brand/Model: Eppendorf Mastercycler X50s
  • Function: DNA amplification using PCR
  • Sample: Purified DNA template ready for PCR
  • Sample Preparation: Prepared by the user; DNA extraction and purification are not included
  • PCR Reagents: Provided by the user, including DNA template, primers, PCR master mix, and other required reaction components
  • PCR Parameters: Denaturation, annealing, extension, number of cycles, final extension, and other conditions are determined by the user
  • Service Output: Amplified PCR product
  • Operation: Performed by the user according to the applicable SOP and under laboratory staff supervision
Conditions of Use
  1. The user must provide information regarding the sample type, target DNA, and PCR program to be used.
  2. The DNA template must be previously extracted and purified and suitable for PCR amplification.
  3. All required PCR reagents and consumables must be provided by the user unless otherwise specified by the laboratory.
  4. The concentration and quality of the DNA template should be appropriate for the intended PCR application.
  5. PCR tubes or plates must be compatible with the Eppendorf Mastercycler X50s.
  6. The PCR program, including denaturation, annealing, extension, number of cycles, and final extension, must be specified by the user.
  7. Appropriate procedures must be followed to prevent cross-contamination between samples.
  8. The work area and equipment must be kept clean before and after use.
  9. PCR products must be handled and disposed of according to the applicable laboratory procedures.
  10. Users must follow all applicable SOPs, laboratory safety procedures, and equipment operating guidelines.
  11. The user is responsible for ensuring the quality, suitability, and safety of the samples and reagents used.


File Name File Size
Service SOP/Form file 0.55 MB

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