Single Particle Reconstruction
Single Particle Reconstruction
Paid


SERVICE DESCRIPTION |    Technical Guidance and Account Setup Service for Single Particle Reconstruction (CryoSPARC) – ELSA BRIN This service is intended for users conducting Single Particle Reconstruction (SPR) analysis using the CryoSPARC platform at BRIN's…

  • Laboratorium Fisika dan Instrumen Pencitraan Maju - Cryo-EM resolusi tinggi
  • KST Cibinong (Soekarno)
    Gedung Genomik Lantai 1 Jl Raya Bogor Km 46 Cibinong - Bogor Jawa Barat
  • 08119811578
  • cryo-em@brin.go.id
  • Service Unit: contract
  • Service Duration: 1 Working Days
  • Service Quota Per Day: 1
  • Service Capacity: 1 contract
  • Minimum Amount: 1
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   Technical Guidance and Account Setup Service for Single Particle Reconstruction (CryoSPARC) – ELSA BRIN

This service is intended for users conducting Single Particle Reconstruction (SPR) analysis using the CryoSPARC platform at BRIN's Electron Microscopy facilities (KRIOS and TUNDRA).

Participant Requirements

  1. Users are encouraged to have Cryo-EM acquisition data from BRIN's CryoEM Laboratory (either from the KRIOS or TUNDRA microscope).

  2. Users must possess or register for a CryoSPARC license, which can be officially obtained via: 🔗CryoSPARC Download Page

Scope of Technical Guidance

  1. Creation of a CryoSPARC user account on the ELSA BRIN server

  2. Installation and configuration of CryoSPARC, including GPU integration and user data storage setup

  3. Introduction to the CryoSPARC interface and project setup (project directory, workspace, and data import)

  4. Basic Single Particle Analysis (SPA) workflow, including:

    • Data import and pre-processing (motion correction and CTF estimation)

    • Particle picking and extraction

    • 2D classification

    • Initial model generation

    • 3D refinement

Objectives

To provide technical understanding and hands-on support for researchers to:

  • Independently manage CryoSPARC accounts and data

  • Efficiently perform basic Single Particle Analysis using CryoEM data

Service Output

  • A fully activated CryoSPARC user account ready for use

  • User comprehension of the fundamental workflow for Single Particle Reconstruction on the CryoSPARC platform



Application Requirements:
  • Photo
  • Other Supporting File
File Name File Size
Service SOP/Form file 0.02 MB
Template Photo 0 MB
Template Other Supporting File 0 MB

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